PrimePCR™ SYBR® Green Assay: GLI3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: GLI3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GLI3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0001509
Assay Design:   Exonic
Chromosome Location:   7:42187992-42262745question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000395925 ENST00000448703

Gene Information

This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases including Greig cephalopolysyndactyly syndrome Pallister-Hall syndrome preaxial polydactyly type IV and postaxial polydactyly types A1 and B. [provided by RefSeq Jul 2008]

Gene Symbol:   GLI3
Gene Name:   GLI family zinc finger 3
Aliases:   ACLS, GCPS, GLI3-190, GLI3FL, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV
RefSeq:   NC_000007.13 NG_008434.1 NT_007819.17
Ensembl:   ENSG00000106571
Entrez:   2737
Chromosome Mapping:   7p13

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