PrimePCR™ SYBR® Green Assay: CD3D, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: CD3D, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CD3D, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0002652
Assay Design:   Exonic
Chromosome Location:   11:118211175-118211282question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000300692 ENST00000534687 ENST00000392884

Gene Information

The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex and along with four other CD3 subunits binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq Feb 2009]

Gene Symbol:   CD3D
Gene Name:   CD3d molecule, delta (CD3-TCR complex)
Aliases:   CD3-DELTA, T3D
RefSeq:   NC_000011.9 NG_007566.1 NG_009891.1 NT_033899.8
Ensembl:   ENSG00000167286
Entrez:   915
Chromosome Mapping:   11q23

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