PrimePCR™ SYBR® Green Assay: CD59, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: CD59, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CD59, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004363
Assay Design:   Exonic
Chromosome Location:   11:33731629-33731728question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000395850 ENST00000533403 ENST00000351554 ENST00000415002 ENST00000445143 ENST00000426650 ENST00000437761 ENST00000527577 ENST00000528700

Gene Information

This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex whereby it binds complement C8 and/or C9 during the assembly of this complex thereby inhibiting the incorporation of multiple copies of C9 into the complex which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency a disease resulting in hemolytic anemia and thrombosis and which causes cerebral infarction. Multiple alternatively spliced transcript variants which encode the same protein have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CD59
Gene Name:   CD59 molecule, complement regulatory protein
Aliases:   16.3A5, 1F5, EJ16, EJ30, EL32, FLJ38134, FLJ92039, G344, HRF-20, HRF20, MAC-IP, MACIF, MEM43, MGC2354, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20
RefSeq:   NC_000011.9 NT_009237.18 NG_008057.1
Ensembl:   ENSG00000085063
Entrez:   966
Chromosome Mapping:   11p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.520000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download