PrimePCR™ SYBR® Green Assay: NTF4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: NTF4, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: NTF4, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0004940
Assay Design:		Exonic
Chromosome Location:		19:49564781-49564969question
Amplicon Length:		159
Splice Variants Targeted:		ENST00000301411

Gene Information

This gene is a member of a family of neurotrophic factors neurotrophins that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor brain-derived neurotrophic factor and neurotrophin 3 prove lethal during early postnatal development NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood. [provided by RefSeq Jul 2008]

Gene Symbol:		NTF4
Gene Name:		neurotrophin 4
Aliases:		GLC10, GLC1O, NT-4/5, NT4, NT5, NTF5
RefSeq:		NC_000019.9 NG_000019.3 NG_016289.1 NT_011109.16
Ensembl:		ENSG00000167744
Entrez:		4909
Chromosome Mapping:		19q13.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	34.660000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.