PrimePCR™ SYBR® Green Assay: IGF2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: IGF2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IGF2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0005504
Assay Design:   Exonic
Chromosome Location:   11:2150707-2150887question
Amplicon Length:   151
Splice Variants Targeted:   ENST00000381395 ENST00000381406 ENST00000416167 ENST00000300632 ENST00000381319

Gene Information

This gene encodes a member of the insulin family of polypeptide growth factors which are involved in development and growth. It is an imprinted gene expressed only from the paternal allele and epigenetic changes at this locus are associated with Wilms tumour Beckwith-Wiedemann syndrome rhabdomyosarcoma and Silver-Russell syndrome. A read-through INS-IGF2 gene exists whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2010]

Gene Symbol:   IGF2
Gene Name:   insulin-like growth factor 2 (somatomedin A)
Aliases:   C11orf43, FLJ22066, FLJ44734, IGF-II, PP9974
RefSeq:   NC_000011.9 NG_008849.1 NT_009237.18
Ensembl:   ENSG00000167244
Entrez:   3481
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.720000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download