PrimePCR™ SYBR® Green Assay: NOTCH2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best specificity

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PrimePCR™ PreAmp for SYBR® Green Assay: NOTCH2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NOTCH2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0005739
Assay Design:   Exonic
Chromosome Location:   1:120496202-120496303question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000256646 ENST00000539617

Gene Information

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats and an intracellular domain consisting of multiple different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia notch interaction with its cell-bound ligands (delta serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands and may play a role in vascular renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jan 2011]

Gene Symbol:   NOTCH2
Gene Name:   notch 2
Aliases:   AGS2, hN2
RefSeq:   NC_000001.10 NG_008163.1 NT_032977.9
Ensembl:   ENSG00000134250
Entrez:   4853
Chromosome Mapping:   1p13-p11

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