PrimePCR™ SYBR® Green Assay: MIF, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0041153


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PrimePCR™ PreAmp for SYBR® Green Assay: MIF, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: MIF, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0038431
Assay Design:		exonic
Chromosome Location:		22:24236663-24236754question
Amplicon Length:		62
Splice Variants Targeted:		ENST00000173229 ENST00000538852 ENST00000546090 ENST00000329962 ENST00000294016 ENST00000359651 ENST00000367284 ENST00000215754 ENST00000406213 ENST00000366787

Gene Information

This gene encodes a lymphokine involved in cell-mediated immunity immunoregulation and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane which may indicate an additional role in integrin signaling pathways. [provided by RefSeq Jul 2008]

Gene Symbol:		MIF
Gene Name:		macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Aliases:		GIF, GLIF, MMIF
RefSeq:		NC_000022.10 NG_012099.1 NT_011520.12
Ensembl:		ENSG00000240972
Entrez:		4282
Chromosome Mapping:		22q11.23

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	36.620000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.