PrimePCR™ SYBR® Green Assay: C1QA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0041247


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PrimePCR™ PreAmp for SYBR® Green Assay: C1QA, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: C1QA, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0038525
Assay Design:		exonic
Chromosome Location:		1:22965557-22965646question
Amplicon Length:		60
Splice Variants Targeted:		ENST00000374642 ENST00000438241 ENST00000402322

Gene Information

This gene encodes a major constituent of the human complement subcomponent C1q. C1q associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains six B-chains and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A- B- and C-chains are arranged in the order A-C-B on chromosome 1. This gene encodes the A-chain polypeptide of human complement subcomponent C1q. [provided by RefSeq Jul 2008]

Gene Symbol:		C1QA
Gene Name:		complement component 1, q subcomponent, A chain
Aliases:		Not Available
RefSeq:		NC_000001.10 NG_007282.1 NG_007565.1 NT_004610.19
Ensembl:		ENSG00000173372
Entrez:		712
Chromosome Mapping:		1p36.12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	35.440000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.