PrimePCR™ SYBR® Green Assay: NFKB2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0049657


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PrimePCR™ PreAmp for SYBR® Green Assay: NFKB2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: NFKB2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042395
Assay Design:		exonic
Chromosome Location:		10:104154513-104155734question
Amplicon Length:		79
Splice Variants Targeted:		ENST00000601386 ENST00000369966 ENST00000471698 ENST00000189444 ENST00000428099

Gene Information

This gene encodes one of the NF-kappaB family of transcription factors which are characterized by a Rel homology domain (RHD) in the N-terminal region. These transcription factors have key roles in regulating immunity stress responses apoptosis and differentiation. The p100 form of this gene is processed in the proteasome to the p52 form. NF-kappaB molecules containing p52 participate in noncanonical NF-kappaB regulatory pathways. Recent reviews of the NF-kappaB signalling pathways include PubMed 17072323 18267068 18515173 and 21772278. [provided by RefSeq Aug 2011]

Gene Symbol:		NFKB2
Gene Name:		nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Aliases:		LYT-10, LYT10, p52
RefSeq:		NC_000010.10 NT_030059.13
Ensembl:		ENSG00000077150
Entrez:		4791
Chromosome Mapping:		10q24

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999900
y-intercept	36.430000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.