PrimePCR™ SYBR® Green Assay: TYR, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0049664


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PrimePCR™ PreAmp for SYBR® Green Assay: TYR, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: TYR, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042402
Assay Design:		exonic
Chromosome Location:		11:88911066-88911209question
Amplicon Length:		114
Splice Variants Targeted:		ENST00000263321

Gene Information

The enzyme encoded by this gene catalyzes the first 2 steps and at least 1 subsequent step in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities and requires copper for function. Mutations in this gene result in oculocutaneous albinism and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene. [provided by RefSeq Oct 2008]

Gene Symbol:		TYR
Gene Name:		tyrosinase (oculocutaneous albinism IA)
Aliases:		CMM8, OCA1A, OCAIA, SHEP3
RefSeq:		NC_000011.9 NG_008748.1 NT_167190.1
Ensembl:		ENSG00000077498
Entrez:		7299
Chromosome Mapping:		11q14-q21

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999900
y-intercept	35.820000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.