PrimePCR™ SYBR® Green Assay: THPO, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0049874


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PrimePCR™ PreAmp for SYBR® Green Assay: THPO, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: THPO, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042612
Assay Design:		exonic
Chromosome Location:		3:184090191-184090340question
Amplicon Length:		120
Splice Variants Targeted:		ENST00000204615 ENST00000445696 ENST00000421442

Gene Information

Megakaryocytopoiesis is the cellular development process that leads to platelet production. The protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL the product of myeloproliferative leukemia virus oncogene. Alternate splicing results in multiple transcript variants of this gene.[provided by RefSeq Apr 2010]

Gene Symbol:		THPO
Gene Name:		thrombopoietin
Aliases:		MGC163194, MGDF, MKCSF, ML, MPLLG, TPO
RefSeq:		NC_000003.11 NG_012136.1 NT_005612.16
Ensembl:		ENSG00000090534
Entrez:		7066
Chromosome Mapping:		3q27

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	36.520000
Efficiency	95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.