PrimePCR™ SYBR® Green Assay: NRCAM, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0049888


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PrimePCR™ PreAmp for SYBR® Green Assay: NRCAM, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: NRCAM, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042626
Assay Design:		exonic
Chromosome Location:		7:107872879-107875029question
Amplicon Length:		61
Splice Variants Targeted:		ENST00000315150 ENST00000393304 ENST00000393308 ENST00000393307 ENST00000393305 ENST00000418265 ENST00000306390 ENST00000341446 ENST00000375820 ENST00000397198 ENST00000367112 ENST00000351718 ENST00000379024 ENST00000425651 ENST00000417701 ENST00000442580 ENST00000419936 ENST00000456431 ENST00000379028 ENST00000413765 ENST00000379022 ENST00000232766 ENST00000219478 ENST00000272164

Gene Information

Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:		NRCAM
Gene Name:		neuronal cell adhesion molecule
Aliases:		KIAA0343, MGC138845, MGC138846
RefSeq:		NC_000007.13 NT_007933.15
Ensembl:		ENSG00000091129
Entrez:		4897
Chromosome Mapping:		7q31

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999900
y-intercept	36.050000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.