PrimePCR™ SYBR® Green Assay: MSH2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0049938


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PrimePCR™ PreAmp for SYBR® Green Assay: MSH2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: MSH2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0042676
Assay Design:		exonic
Chromosome Location:		2:47637293-47637393question
Amplicon Length:		71
Splice Variants Targeted:		ENST00000233146 ENST00000454849 ENST00000406134 ENST00000543555

Gene Information

MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned it was discovered to be a human homolog of the E. coli mismatch repair gene mutS consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. [provided by RefSeq Jul 2008]

Gene Symbol:		MSH2
Gene Name:		mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
Aliases:		COCA1, FCC1, HNPCC, HNPCC1, LCFS2
RefSeq:		NC_000002.11 NG_007110.1 NT_022184.15
Ensembl:		ENSG00000095002
Entrez:		4436
Chromosome Mapping:		2p21

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999900
y-intercept	35.780000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.