PrimePCR™ SYBR® Green Assay: CSF3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0050480


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PrimePCR™ PreAmp for SYBR® Green Assay: CSF3, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CSF3, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0043218
Assay Design:		exonic
Chromosome Location:		17:38171964-38172097question
Amplicon Length:		104
Splice Variants Targeted:		ENST00000302259 ENST00000558850 ENST00000322329 ENST00000526775 ENST00000377627 ENST00000377577 ENST00000583218 ENST00000394149 ENST00000225474 ENST00000331769 ENST00000394148 ENST00000577675 ENST00000265562 ENST00000431726 ENST00000307017 ENST00000278353 ENST00000258243

Gene Information

The protein encoded by this gene is a cytokine that controls the production differentiation and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq May 2010]

Gene Symbol:		CSF3
Gene Name:		colony stimulating factor 3 (granulocyte)
Aliases:		C17orf33, CSF3OS, GCSF, MGC45931
RefSeq:		NC_000017.10 NT_010783.15
Ensembl:		ENSG00000108342
Entrez:		1440
Chromosome Mapping:		17q11.2-q12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	35.950000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.