PrimePCR™ SYBR® Green Assay: RAP1A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0050946

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RAP1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RAP1A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043684
Assay Design:   exonic
Chromosome Location:   1:112162543-112234035question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000352645 ENST00000351589 ENST00000330339 ENST00000559187 ENST00000330244 ENST00000561157 ENST00000356415 ENST00000433097 ENST00000369709 ENST00000436150 ENST00000545460 ENST00000343110 ENST00000378078 ENST00000484157 ENST00000303343 ENST00000368404 ENST00000368401 ENST00000270800

Gene Information

The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   RAP1A
Gene Name:   RAP1A, member of RAS oncogene family
Aliases:   KREV-1, KREV1, RAP1, SMGP21
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000116473
Entrez:   5906
Chromosome Mapping:   1p13.3

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