PrimePCR™ SYBR® Green Assay: NCAPH, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051157


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PrimePCR™ PreAmp for SYBR® Green Assay: NCAPH, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: NCAPH, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0043895
Assay Design:		exonic
Chromosome Location:		2:97009949-97017675question
Amplicon Length:		96
Splice Variants Targeted:		ENST00000240423 ENST00000427946 ENST00000435975 ENST00000456906 ENST00000455200

Gene Information

This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes except during the early phase of chromosome condensation. During interphase the protein has a distinct punctate nucleolar localization. [provided by RefSeq Jul 2008]

Gene Symbol:		NCAPH
Gene Name:		non-SMC condensin I complex, subunit H
Aliases:		BRRN1, CAP-H, HCAP-H
RefSeq:		NC_000002.11 NT_022171.15
Ensembl:		ENSG00000121152
Entrez:		23397
Chromosome Mapping:		2q11.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999400
y-intercept	35.630000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.