PrimePCR™ SYBR® Green Assay: ENPP3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0052891

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ENPP3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ENPP3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045630
Assay Design:   exonic
Chromosome Location:   6:131973719-131973979question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000414305 ENST00000357639 ENST00000358229 ENST00000543135 ENST00000427148

Gene Information

The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas small intestine colon and liver. The human mRNA is expressed in glioma cells prostate and uterus. Expression of the human protein has been detected in uterus basophils and mast cells. [provided by RefSeq Jul 2008]

Gene Symbol:   ENPP3
Gene Name:   ectonucleotide pyrophosphatase/phosphodiesterase 3
Aliases:   B10, CD203c, NPP3, PD-IBETA, PDNP3
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000154269
Entrez:   5169
Chromosome Mapping:   6q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.950000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download