PrimePCR™ SYBR® Green Assay: CALM3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053133


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PrimePCR™ PreAmp for SYBR® Green Assay: CALM3, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CALM3, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0045872
Assay Design:		exonic
Chromosome Location:		19:47111755-47112111question
Amplicon Length:		70
Splice Variants Targeted:		ENST00000391918 ENST00000291295 ENST00000598871 ENST00000594523 ENST00000599839 ENST00000596362 ENST00000557020 ENST00000356978 ENST00000447653 ENST00000553542 ENST00000544280 ENST00000272298 ENST00000456319 ENST00000409563

Gene Information

Description Not Available

Gene Symbol:		CALM3
Gene Name:		calmodulin 3 (phosphorylase kinase, delta)
Aliases:		CALM1, CALM2, PHKD, PHKD3
RefSeq:		NC_000019.9 NT_011109.16
Ensembl:		ENSG00000160014
Entrez:		808
Chromosome Mapping:		19q13.2-q13.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999100
y-intercept	36.350000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.