PrimePCR™ SYBR® Green Assay: REL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0053286


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PrimePCR™ PreAmp for SYBR® Green Assay: REL, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: REL, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0046025
Assay Design:		exonic
Chromosome Location:		2:61149284-61149419question
Amplicon Length:		106
Splice Variants Targeted:		ENST00000295025 ENST00000394479

Gene Information

The REL gene encodes c-Rel a transcription factor that is a member of the Rel/NFKB family which also includes RELA (MIM 164014) RELB (604758) NFKB1 (MIM 164011) and NFKB2 (MIM 164012). These proteins are related through a highly conserved N-terminal region termed the 'Rel domain' which is responsible for DNA binding dimerization nuclear localization and binding to the NFKB inhibitor (MIM 164008) (Belguise and Sonenshein 2007 [PubMed 18037997]).[supplied by OMIM May 2008]

Gene Symbol:		REL
Gene Name:		v-rel reticuloendotheliosis viral oncogene homolog (avian)
Aliases:		C-Rel
RefSeq:		NC_000002.11 NT_022184.15
Ensembl:		ENSG00000162924
Entrez:		5966
Chromosome Mapping:		2p13-p12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999100
y-intercept	36.310000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.