PrimePCR™ SYBR® Green Assay: A2M, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0054402


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PrimePCR™ PreAmp for SYBR® Green Assay: A2M, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: A2M, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0047141
Assay Design:		exonic
Chromosome Location:		12:9265023-9265124question
Amplicon Length:		72
Splice Variants Targeted:		ENST00000318602 ENST00000404455

Gene Information

Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases including trypsin thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta the major component of beta-amyloid deposits. [provided by RefSeq Jul 2008]

Gene Symbol:		A2M
Gene Name:		alpha-2-macroglobulin
Aliases:		CPAMD5, DKFZp779B086, S863-7
RefSeq:		NC_000012.11 NG_011717.1 NT_009714.17
Ensembl:		ENSG00000175899
Entrez:		2
Chromosome Mapping:		12p13.31

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999300
y-intercept	35.740000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.