PrimePCR™ SYBR® Green Assay: USP18, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054966

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: USP18, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: USP18, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047706
Assay Design:   exonic
Chromosome Location:   22:18659595-18659716question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000215794

Gene Information

The protein encoded by this gene belongs to the ubiquitin-specific proteases (UBP) family of enzymes that cleave ubiquitin from ubiquitinated protein substrates. It is highly expressed in liver and thymus and is localized to the nucleus. This protein efficiently cleaves only ISG15 (a ubiquitin-like protein) fusions and deletion of this gene in mice results in a massive increase of ISG15 conjugates in tissues indicating that this protein is a major ISG15-specific protease. Mice lacking this gene are also hypersensitive to interferon suggesting a function of this protein in downregulating interferon responses independent of its isopeptidase activity towards ISG15. [provided by RefSeq Sep 2011]

Gene Symbol:   USP18
Gene Name:   ubiquitin specific peptidase 18
Aliases:   ISG43, UBP43
RefSeq:   NC_000022.10 NT_011519.10
Ensembl:   ENSG00000184979
Entrez:   11274
Chromosome Mapping:   22q11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.280000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download