PrimePCR™ SYBR® Green Assay: CEBPA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0056075


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PrimePCR™ PreAmp for SYBR® Green Assay: CEBPA, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CEBPA, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0048815
Assay Design:		exonic
Chromosome Location:		19:33791930-33792077question
Amplicon Length:		118
Splice Variants Targeted:		ENST00000498907

Gene Information

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin a protein that plays an important role in body weight homeostasis. Also the encoded protein can interact with CDK2 and CDK4 thereby inhibiting these kinases and causing growth arrest in cultured cells. [provided by RefSeq Jul 2008]

Gene Symbol:		CEBPA
Gene Name:		CCAAT/enhancer binding protein (C/EBP), alpha
Aliases:		C/EBP-alpha, CEBP
RefSeq:		NC_000019.9 NG_012022.1 NT_011109.16
Ensembl:		ENSG00000245848
Entrez:		1050
Chromosome Mapping:		19q13.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.850000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.