PrimePCR™ SYBR® Green Assay: LDHA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0057534


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PrimePCR™ PreAmp for SYBR® Green Assay: LDHA, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: LDHA, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0056429
Assay Design:		exonic
Chromosome Location:		11:18421091-18422476question
Amplicon Length:		68
Splice Variants Targeted:		ENST00000422447 ENST00000543445 ENST00000396222 ENST00000227157 ENST00000478970 ENST00000495052 ENST00000379412 ENST00000542179 ENST00000540430 ENST00000541669 ENST00000280704 ENST00000537486 ENST00000544105 ENST00000535809

Gene Information

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq Sep 2008]

Gene Symbol:		LDHA
Gene Name:		lactate dehydrogenase A
Aliases:		GSD11, LDH1, LDHM
RefSeq:		NC_000011.9 NG_011816.1 NT_009237.18 NG_008185.1
Ensembl:		ENSG00000134333
Entrez:		3939
Chromosome Mapping:		11p15.4

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999000
y-intercept	36.380000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.