PrimePCR™ SYBR® Green Assay: DUSP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0058468

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DUSP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DUSP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0057363
Assay Design:   exonic
Chromosome Location:   5:172195316-172195441question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000239223

Gene Information

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq Jul 2008]

Gene Symbol:   DUSP1
Gene Name:   dual specificity phosphatase 1
Aliases:   CL100, HVH1, MKP-1, MKP1, PTPN10
RefSeq:   NC_000005.9 NT_023133.13
Ensembl:   ENSG00000120129
Entrez:   1843
Chromosome Mapping:   5q34

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