PrimePCR™ SYBR® Green Assay: IL12RB2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0026196


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PrimePCR™ PreAmp for SYBR® Green Assay: IL12RB2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: IL12RB2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0006511
Assay Design:		Intron-spanning
Chromosome Location:		1:67794016-67795389question
Amplicon Length:		142
Splice Variants Targeted:		ENST00000262345 ENST00000441640 ENST00000544434 ENST00000371000 ENST00000541374

Gene Information

The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases such as Crohn's disease and leprosy which is thought to contribute to the inflammatory response and host defense. [provided by RefSeq Jul 2008]

Gene Symbol:		IL12RB2
Gene Name:		interleukin 12 receptor, beta 2
Aliases:		Not Available
RefSeq:		NC_000001.10 NT_032977.9
Ensembl:		ENSG00000081985
Entrez:		3595
Chromosome Mapping:		1p31.3-p31.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	34.920000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.