PrimePCR™ SYBR® Green Assay: ANXA5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026996

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ANXA5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ANXA5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008929
Assay Design:   Intron-spanning
Chromosome Location:   4:122591116-122592765question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000296511 ENST00000512232 ENST00000501272 ENST00000515017

Gene Information

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction inflammation growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I vascular anticoagulant-alpha endonexin II lipocortin V placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq Jul 2008]

Gene Symbol:   ANXA5
Gene Name:   annexin A5
Aliases:   ANX5, ENX2, PP4
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000164111
Entrez:   308
Chromosome Mapping:   4q26-q28,4q27

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 34.370000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download