PrimePCR™ SYBR® Green Assay: IL1R1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0027424


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PrimePCR™ PreAmp for SYBR® Green Assay: IL1R1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: IL1R1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0010015
Assay Design:		Intron-spanning
Chromosome Location:		2:102782647-102788268question
Amplicon Length:		138
Splice Variants Targeted:		ENST00000409929 ENST00000424272 ENST00000409329 ENST00000428279 ENST00000409288 ENST00000410023 ENST00000233946

Gene Information

The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A) interleukin beta (IL1B) and interleukin 1 receptor type I(IL1R1/IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor type II (IL1R2) interleukin 1 receptor-like 2 (IL1RL2) and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. [provided by RefSeq Jul 2008]

Gene Symbol:		IL1R1
Gene Name:		interleukin 1 receptor, type I
Aliases:		CD121A, D2S1473, IL-1R-alpha, IL1R, IL1RA, P80
RefSeq:		NC_000002.11 NT_022171.15
Ensembl:		ENSG00000115594
Entrez:		3554
Chromosome Mapping:		2q12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.995700
y-intercept	33.960000
Efficiency	105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.