PrimePCR™ SYBR® Green Assay: IL17F, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0027955


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PrimePCR™ PreAmp for SYBR® Green Assay: IL17F, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: IL17F, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0011295
Assay Design:		Intron-spanning
Chromosome Location:		6:52103700-52109302question
Amplicon Length:		127
Splice Variants Targeted:		ENST00000336123

Gene Information

The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells and has been shown to stimulate the production of several other cytokines including IL6 IL8 and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2 TGFB1/TGFB and monocyte chemoattractant protein-1. [provided by RefSeq Jul 2008]

Gene Symbol:		IL17F
Gene Name:		interleukin 17F
Aliases:		IL-17F, ML-1, ML1
RefSeq:		NC_000006.11 NT_007592.15
Ensembl:		ENSG00000112116
Entrez:		112744
Chromosome Mapping:		6p12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.997600
y-intercept	35.510000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.