PrimePCR™ SYBR® Green Assay: APOA1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: APOA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: APOA1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012632
Assay Design:   Intron-spanning
Chromosome Location:   11:116707101-116707833question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000375320 ENST00000359492 ENST00000375323 ENST00000236850

Gene Information

This gene encodes apolipoprotein A-I which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies including Tangier disease and with systemic non-neuropathic amyloidosis. [provided by RefSeq Jul 2008]

Gene Symbol:   APOA1
Gene Name:   apolipoprotein A-I
Aliases:   MGC117399
RefSeq:   NC_000011.9 NG_012021.1 NT_033899.8
Ensembl:   ENSG00000118137
Entrez:   335
Chromosome Mapping:   11q23-q24

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