PrimePCR™ SYBR® Green Assay: CD79B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0029249


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PrimePCR™ PreAmp for SYBR® Green Assay: CD79B, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CD79B, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0014190
Assay Design:		Intron-spanning
Chromosome Location:		17:62007701-62009644question
Amplicon Length:		156
Splice Variants Targeted:		ENST00000392795 ENST00000006750

Gene Information

The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins Ig-alpha and Ig-beta which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:		CD79B
Gene Name:		CD79b molecule, immunoglobulin-associated beta
Aliases:		AGM6, B29, IGB
RefSeq:		NC_000017.10 NT_010783.15 NG_007368.1
Ensembl:		ENSG00000007312
Entrez:		974
Chromosome Mapping:		17q23

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998800
y-intercept	35.440000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.