PrimePCR™ SYBR® Green Assay: ARHGAP24, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0029521


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PrimePCR™ PreAmp for SYBR® Green Assay: ARHGAP24, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: ARHGAP24, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0014705
Assay Design:		Intron-spanning
Chromosome Location:		4:86863366-86893283question
Amplicon Length:		126
Splice Variants Targeted:		ENST00000395184 ENST00000503995 ENST00000395183 ENST00000509300 ENST00000514229 ENST00000264343

Gene Information

ARHGAPs such as ARHGAP24 encode negative regulators of Rho GTPases (see ARHA; MIM 165390) which are implicated in actin remodeling cell polarity and cell migration (Katoh and Katoh 2004 [PubMed 15254788]).[supplied by OMIM Mar 2008]

Gene Symbol:		ARHGAP24
Gene Name:		Rho GTPase activating protein 24
Aliases:		DKFZp564B1162, FILGAP, FLJ33877, RC-GAP72, RCGAP72, p73, p73RhoGAP
RefSeq:		NC_000004.11 NT_016354.19
Ensembl:		ENSG00000138639
Entrez:		83478
Chromosome Mapping:		4q22.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	34.910000
Efficiency	102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.