PrimePCR™ SYBR® Green Assay: SMAD4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030084

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SMAD4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SMAD4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0015670
Assay Design:   Intron-spanning
Chromosome Location:   18:48573582-48575111question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000398417 ENST00000342988 ENST00000544926 ENST00000452201

Gene Information

This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer juvenile polyposis syndrome and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq Oct 2009]

Gene Symbol:   SMAD4
Gene Name:   SMAD family member 4
Aliases:   DPC4, JIP, MADH4
RefSeq:   NC_000018.9 NG_013013.1 NT_010966.14
Ensembl:   ENSG00000141646
Entrez:   4089
Chromosome Mapping:   18q21.1

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