PrimePCR™ SYBR® Green Assay: C5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031459

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: C5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: C5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017789
Assay Design:   Intron-spanning
Chromosome Location:   9:123794396-123797124question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000223642 ENST00000430906

Gene Information

The protein encoded by this gene is the fifth component of complement which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide C5a which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component and this complex is the basis for formation of the membrane attack complex which includes additional complement components. Mutations in this gene cause complement component 5 deficiency a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to a susceptibility to liver fibrosis and to rheumatoid arthritis. [provided by RefSeq Jul 2008]

Gene Symbol:   C5
Gene Name:   complement component 5
Aliases:   CPAMD4, FLJ17816, FLJ17822, MGC142298
RefSeq:   NC_000009.11 NG_007364.1 NT_008470.19
Ensembl:   ENSG00000106804
Entrez:   727
Chromosome Mapping:   9q33-q34

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