PrimePCR™ SYBR® Green Assay: FCER2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0032890


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PrimePCR™ PreAmp for SYBR® Green Assay: FCER2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: FCER2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0021054
Assay Design:		Intron-spanning
Chromosome Location:		19:7755363-7761905question
Amplicon Length:		147
Splice Variants Targeted:		ENST00000346664 ENST00000360067

Gene Information

The protein encoded by this gene is a B-cell specific antigen and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation and the regulation of IgE production. This protein also exists as a soluble secreted form then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq Jul 2011]

Gene Symbol:		FCER2
Gene Name:		Fc fragment of IgE, low affinity II, receptor for (CD23)
Aliases:		CD23, CD23A, CLEC4J, FCE2, IGEBF
RefSeq:		NC_000019.9 NT_077812.2
Ensembl:		ENSG00000104921
Entrez:		2208
Chromosome Mapping:		19p13.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999900
y-intercept	34.540000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.