PrimePCR™ SYBR® Green Assay: CYP2C8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0033001


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PrimePCR™ PreAmp for SYBR® Green Assay: CYP2C8, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CYP2C8, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0021321
Assay Design:		Intron-spanning
Chromosome Location:		10:96802761-96805641question
Amplicon Length:		119
Splice Variants Targeted:		ENST00000371270 ENST00000535898 ENST00000535868 ENST00000539050

Gene Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize many xenobiotics including the anticonvulsive drug mephenytoin benzo(a)pyrene 7-ethyoxycoumarin and the anti-cancer drug taxol. This gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq Nov 2010]

Gene Symbol:		CYP2C8
Gene Name:		cytochrome P450, family 2, subfamily C, polypeptide 8
Aliases:		CPC8, CYPIIC8, MP-12/MP-20
RefSeq:		NC_000010.10 NG_007972.1 NT_030059.13
Ensembl:		ENSG00000138115
Entrez:		1558
Chromosome Mapping:		10q23.33

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999400
y-intercept	35.080000
Efficiency	96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.