PrimePCR™ SYBR® Green Assay: SEC24D, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0033042


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PrimePCR™ PreAmp for SYBR® Green Assay: SEC24D, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SEC24D, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0021427
Assay Design:		Intron-spanning
Chromosome Location:		4:119666159-119673868question
Amplicon Length:		137
Splice Variants Targeted:		ENST00000280551 ENST00000511481 ENST00000379735 ENST00000429811 ENST00000419654

Gene Information

The protein encoded by this gene is a member of the SEC24 subfamily of the SEC23/SEC24 family which is involved in vesicle trafficking. The encoded protein has similarity to yeast Sec24p component of COPII. COPII is the coat protein complex responsible for vesicle budding from the ER. This gene product is implicated in the shaping of the vesicle and also in cargo selection and concentration. [provided by RefSeq Jul 2008]

Gene Symbol:		SEC24D
Gene Name:		SEC24 family, member D (S. cerevisiae)
Aliases:		FLJ43974, KIAA0755
RefSeq:		NC_000004.11 NT_016354.19
Ensembl:		ENSG00000150961
Entrez:		9871
Chromosome Mapping:		4q26

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998800
y-intercept	34.770000
Efficiency	95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.