PrimePCR™ SYBR® Green Assay: GSS, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0033248


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PrimePCR™ PreAmp for SYBR® Green Assay: GSS, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: GSS, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0021949
Assay Design:		Intron-spanning
Chromosome Location:		20:33530742-33533836question
Amplicon Length:		119
Splice Variants Targeted:		ENST00000216951 ENST00000541098

Gene Information

Glutathione is important for a variety of biological functions including protection of cells from oxidative damage by free radicals detoxification of xenobiotics and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq Jul 2008]

Gene Symbol:		GSS
Gene Name:		glutathione synthetase
Aliases:		GSHS, MGC14098
RefSeq:		NC_000020.10 NG_008848.1 NG_011520.1 NG_016984.1 NT_011362.10
Ensembl:		ENSG00000100983
Entrez:		2937
Chromosome Mapping:		20q11.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	34.360000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.