PrimePCR™ SYBR® Green Assay: SHC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0033445


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PrimePCR™ PreAmp for SYBR® Green Assay: SHC1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SHC1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0022485
Assay Design:		Intron-spanning
Chromosome Location:		1:154938959-154940260question
Amplicon Length:		119
Splice Variants Targeted:		ENST00000368445 ENST00000448116 ENST00000368449 ENST00000368453 ENST00000368450 ENST00000414115 ENST00000444179 ENST00000368443 ENST00000368441

Gene Information

This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms p52Shc and p46Shc link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Feb 2011]

Gene Symbol:		SHC1
Gene Name:		SHC (Src homology 2 domain containing) transforming protein 1
Aliases:		FLJ26504, SHC, SHCA
RefSeq:		NC_000001.10 NT_004487.19
Ensembl:		ENSG00000160691
Entrez:		6464
Chromosome Mapping:		1q21

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.997700
y-intercept	34.960000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.