PrimePCR™ SYBR® Green Assay: COPZ1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0041291


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PrimePCR™ PreAmp for SYBR® Green Assay: COPZ1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: COPZ1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0038569
Assay Design:		Intron-spanning
Chromosome Location:		12:54736011-54737059question
Amplicon Length:		82
Splice Variants Targeted:		ENST00000552848 ENST00000262061 ENST00000549043 ENST00000552218 ENST00000553231 ENST00000552362 ENST00000455864 ENST00000551779 ENST00000548753 ENST00000550713

Gene Information

Description Not Available

Gene Symbol:		COPZ1
Gene Name:		coatomer protein complex, subunit zeta 1
Aliases:		COPZ, zeta1-COP
RefSeq:		NC_000012.11 NT_029419.12
Ensembl:		ENSG00000111481
Entrez:		22818
Chromosome Mapping:		12q13.2-q13.3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998800
y-intercept	35.590000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.