PrimePCR™ SYBR® Green Assay: RAB4B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0041667

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RAB4B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RAB4B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0038945
Assay Design:   Intron-spanning
Chromosome Location:   19:41289903-41292805question
Amplicon Length:   197
Splice Variants Targeted:   ENST00000525929 ENST00000293328 ENST00000533721 ENST00000594800 ENST00000602173 ENST00000595728 ENST00000357052 ENST00000491143 ENST00000262095 ENST00000394303 ENST00000226574 ENST00000394820 ENST00000507079 ENST00000332624 ENST00000589578 ENST00000572045 ENST00000337907 ENST00000400908 ENST00000391967 ENST00000367313 ENST00000248996 ENST00000377200 ENST00000340434 ENST00000389722 ENST00000533760 ENST00000316851 ENST00000294309 ENST00000542467 ENST00000262940 ENST00000272117 ENST00000429204 ENST

Gene Information

Description Not Available

Gene Symbol:   RAB4B
Gene Name:   RAB4B, member RAS oncogene family
Aliases:   FLJ78649, MGC52123
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000167578
Entrez:   53916
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.900000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download