PrimePCR™ SYBR® Green Assay: Aph1a, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best specificity; Same primer pair as used in probe assay qMmuCEP0043017

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Aph1a, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Aph1a, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0040800
Assay Design:   exonic
Chromosome Location:   3:95894821-95894912question
Amplicon Length:   62
Splice Variants Targeted:   ENSMUST00000034707 ENSMUST00000174008 ENSMUST00000172996 ENSMUST00000098948 ENSMUST00000086281 ENSMUST00000103121 ENSMUST00000046944 ENSMUST00000169335 ENSMUST00000117662 ENSMUST00000117296 ENSMUST00000154842 ENSMUST00000066994 ENSMUST00000049348 ENSMUST00000142742 ENSMUST00000111308 ENSMUST00000021268 ENSMUST00000088448 ENSMUST00000056710 ENSMUST00000015894 ENSMUST00000017783 ENSMUST00000085774 ENSMUST00000074546

Gene Information

Description Not Available

Gene Symbol:   Aph1a
Gene Name:   Anterior pharynx defective 1a homolog (C. elegans)
Aliases:   6530402N02Rik, APH-1a, Aph-1
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000015750
Entrez:   226548
Chromosome Mapping:   3 F2.1|3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 39.160000
Efficiency 88

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download