PrimePCR™ SYBR® Green Assay: Sp3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qMmuCEP0054504


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PrimePCR™ PreAmp for SYBR® Green Assay: Sp3, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Sp3, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCED0046078
Assay Design:		exonic
Chromosome Location:		2:72970904-72971050question
Amplicon Length:		117
Splice Variants Targeted:		ENSMUST00000102689 ENSMUST00000066003

Gene Information

This gene product belongs to a family of Sp1 related transcription factors which regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains and has been reported to function as a bifunctional transcription factor that either stimulates or represses transcription of numerous genes. Alternative splicing results in transcript variants encoding different isoforms and one variant initiates translation from a non-AUG (AUA) codon. [provided by RefSeq Jul 2008]

Gene Symbol:		Sp3
Gene Name:		Trans-acting transcription factor 3
Aliases:		D130027J01Rik
RefSeq:		NC_000068.7 NT_039207.8
Ensembl:		ENSMUSG00000027109
Entrez:		20687
Chromosome Mapping:		2 C3\|2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.998800
y-intercept	35.180000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.