PrimePCR™ SYBR® Green Assay: Abat, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0059334


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PrimePCR™ PreAmp for SYBR® Green Assay: Abat, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Abat, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCED0050908
Assay Design:		exonic
Chromosome Location:		16:8602290-8603487question
Amplicon Length:		77
Splice Variants Targeted:		ENSMUST00000016172 ENSMUST00000076803 ENSMUST00000028678 ENSMUST00000065987 ENSMUST00000115838 ENSMUST00000115839 ENSMUST00000138987 ENSMUST00000144444 ENSMUST00000113352

Gene Information

The encoded gene product is responsible for catabolism of gamma-aminobutyric acid (GABA) a mostly inhibitory neurotransmitter in the central nervous system into succinic semialdehyde. Deficiency of this encoded protein includes psychomotor retardation hypotonia hyperreflexia lethargy refractory seizures and EEG abnormalities. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jan 2010]

Gene Symbol:		Abat
Gene Name:		4-aminobutyrate aminotransferase
Aliases:		9630038C02Rik, AI255750, ENSMUSG00000051226, Gabaat, Gabat, Gm9851, I54, Laibat, X61497
RefSeq:		NC_000082.6 NT_039624.8
Ensembl:		ENSMUSG00000057880
Entrez:		268860
Chromosome Mapping:		16\|16 A3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	35.310000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.