PrimePCR™ SYBR® Green Assay: Mdc1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Mdc1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Mdc1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0005891
Assay Design:   Intron-spanning
Chromosome Location:   17:35844528-35845902question
Amplicon Length:   77
Splice Variants Targeted:   ENSMUST00000082337 ENSMUST00000174124

Gene Information

The protein encoded by this gene contains an N-terminal forkhead domain two BRCA1 C-terminal (BRCT) motifs and a central domain with 7 divergent copies of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. Mice with mutations in this gene exhibit growth retardation male infertility immune defects chromosome instability DNA repair defects and radiation sensitivity. [provided by RefSeq Jul 2008]

Gene Symbol:   Mdc1
Gene Name:   Mediator of DNA damage checkpoint 1
Aliases:   6820401C03, AA413496, Nfbd1, mKIAA0170
RefSeq:   NC_000083.6 NT_039649.8 NT_187004.1 NT_187027.1
Ensembl:   ENSMUSG00000061607
Entrez:   240087
Chromosome Mapping:   17 B1|17

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