PrimePCR™ SYBR® Green Assay: Tnfrsf12a, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Tnfrsf12a, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Tnfrsf12a, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0002871
Assay Design:   exonic
Chromosome Location:   10:12819437-12819646question
Amplicon Length:   88
Splice Variants Targeted:   ENSRNOT00000004842

Gene Information

human homolog is a receptor that binds TWEAK, a member of the TNF family, and is involved in cell proliferation, adhesion and angiogenesis [RGD, Feb 2006]

Gene Symbol:   Tnfrsf12a
Gene Name:   Tumor necrosis factor receptor superfamily member 12A precursor
Aliases:   Not Available
RefSeq:   NM_181086
Ensembl:   ENSRNOG00000003546
Entrez:   302965
Chromosome Mapping:   10q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.930000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download