PrimePCR™ SYBR® Green Assay: Faslg, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Faslg, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Faslg, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0003046
Assay Design:		exonic
Chromosome Location:		13:84597882-84598692question
Amplicon Length:		95
Splice Variants Targeted:		ENSRNOT00000003998

Gene Information

ligand that binds the Fas receptor; plays a role in induction of apoptosis [RGD, Feb 2006]

Gene Symbol:		Faslg
Gene Name:		Tumor necrosis factor ligand superfamily member 6 Tumor necrosis factor ligand superfamily member 6, membrane form Tumor necrosis factor ligand superfamily member 6, soluble form ADAM10-processed FasL form FasL intracellular domain
Aliases:		Not Available
RefSeq:		NM_012908
Ensembl:		ENSRNOG00000002978
Entrez:		25385
Chromosome Mapping:		13q22

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	36.000000
Efficiency	103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.