PrimePCR™ SYBR® Green Assay: Pde4a, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Pde4a, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Pde4a, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0054148
Assay Design:		Exonic
Chromosome Location:		8:22288702-22288840question
Amplicon Length:		109
Splice Variants Targeted:		ENSRNOT00000028272 ENSRNOT00000061100 ENSRNOT00000061099 ENSRNOT00000074313 ENSRNOT00000041381 ENSRNOT00000012792 ENSRNOT00000029340

Gene Information

human homolog catalyzes the hydrolysis of cAMP; activity is inhibited by rolipram [RGD, Feb 2006]

Gene Symbol:		Pde4a
Gene Name:		CAMP-specific 3',5'-cyclic phosphodiesterase 4A
Aliases:		Not Available
RefSeq:		NM_013101
Ensembl:		ENSRNOG00000020828
Entrez:		25638
Chromosome Mapping:		8q13

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	37.110000
Efficiency	95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.