PrimePCR™ SYBR® Green Assay: MLH1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0049644

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MLH1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MLH1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0042382
Assay Design:   exonic
Chromosome Location:   3:37070305-37081680question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000231790 ENST00000456676 ENST00000458205 ENST00000455445 ENST00000435176 ENST00000450420 ENST00000413740 ENST00000536378 ENST00000539477

Gene Information

This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but their full-length natures have not been determined.[provided by RefSeq Nov 2009]

Gene Symbol:   MLH1
Gene Name:   mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli)
Aliases:   COCA2, FCC2, HNPCC, HNPCC2, MGC5172, hMLH1
RefSeq:   NC_000003.11 NG_007109.1 NG_008418.1 NT_022517.18
Ensembl:   ENSG00000076242
Entrez:   4292
Chromosome Mapping:   3p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.560000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download