PrimePCR™ SYBR® Green Assay: IFNAR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052311

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: IFNAR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IFNAR1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045049
Assay Design:   exonic
Chromosome Location:   21:34707854-34707953question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000270139 ENST00000442071 ENST00000442357 ENST00000416947

Gene Information

The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases which in turn phosphorylate several proteins including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. [provided by RefSeq Jul 2008]

Gene Symbol:   IFNAR1
Gene Name:   interferon (alpha, beta and omega) receptor 1
Aliases:   AVP, IFN-alpha-REC, IFNAR, IFNBR, IFRC
RefSeq:   NC_000021.8 NT_011512.11
Ensembl:   ENSG00000142166
Entrez:   3454
Chromosome Mapping:   21q22.1,21q22.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.860000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download