PrimePCR™ SYBR® Green Assay: RCAN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0033054


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PrimePCR™ PreAmp for SYBR® Green Assay: RCAN1, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: RCAN1, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0021477
Assay Design:		Intron-spanning
Chromosome Location:		21:35890472-35893857question
Amplicon Length:		114
Splice Variants Targeted:		ENST00000487990 ENST00000313806 ENST00000381132 ENST00000399272 ENST00000481448 ENST00000482533 ENST00000381135 ENST00000443408

Gene Information

The protein encoded by this gene interacts with calcineurin A and inhibits calcineurin-dependent signaling pathways possibly affecting central nervous system development. This gene is located in the minimal candidate region for the Down syndrome phenotype and is overexpressed in the brain of Down syndrome fetuses. Chronic overexpression of this gene may lead to neurofibrillary tangles such as those associated with Alzheimer disease. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:		RCAN1
Gene Name:		regulator of calcineurin 1
Aliases:		ADAPT78, CSP1, DSC1, DSCR1, MCIP1, RCN1
RefSeq:		NC_000021.8 NG_007071.1 NT_011512.11
Ensembl:		ENSG00000159200
Entrez:		1827
Chromosome Mapping:		21q22.1-q22.2,21q22.12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.997800
y-intercept	34.750000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.